Rezumat
CZU 544.43; 544.473:577.15; 577.115
DOI https://doi.org/10.5281/zenodo.1054137
It is presented the first part of the theoretical study devoted to the description of the kinetics and mechanism of the lipid peroxidation process involving the complexes of cytochrome c and cardiolipin, taking into account the effect of the antioxidant. The main components of the ROS (reactive oxygen species) and AOD (antioxidant defense) systems and their properties are considered. The key features of the functioning of these systems and various channels of the influence of their components on each other, both intra-systemic and inter-systemic, essential for the optimal interaction of these systems in the organism are discussed. In the review a special attention is paid to the experimental works in which the properties and structure of the cytochrome c and cardiolipin complexes of various types along with the peroxidase activity manifested by them are studied. In addition to two known ways for regulating the peroxidase activity of these complexes, discussed in the literature, it is proposed to take into account another way, which is connected with a possibility of the inclusion of the lipophilic antioxidant molecules in a composition of the complexes under study. The proposed way for regulating the peroxidase activity, increasing the effectiveness of the control over the peroxidase process, opens up new opportunities to regulate the process of the apoptosis of cells. Based on the analysis of experimental works on this problem, a theoretical kinetic model of the peroxidase process is formulated, which includes two reaction pathways – an enzymatic pathway involving the complexes of cytochrome c and cardiolipin, and a non-enzymatic pathway involving free radicals. The system of differential equations that describes the kinetics of the process of lipid peroxidation is constructed with account of the inhibiting effect of the antioxidant. The obtained model system of the kinetic equations will be used to study and compare the antioxidant activity of vitamin E (α-tocopherol) and some of its homologues with a shortened side chain, relying on the available theoretical and experimental data.
Keywords: antioxidant activity, complexes of cytochrome c and cardiolipin, lipid peroxidation, chemical kinetics, free radicals, α-tocopherol.