Purification of Alpha-Amylase Produced by <i>Aspergillus niger</i> CNMN FD 06 under Submerged Cultivation in the Presence of TIO<sub>2</sub> Nanoparticles and the Coordination Compound of Sr(II) with Polydentat Ligand
Vol. 60 No. 6 (2024), Content
Vol. 60 No. 6 (2024)

Purification of Alpha-Amylase Produced by <i>Aspergillus niger</i> CNMN FD 06 under Submerged Cultivation in the Presence of TIO<sub>2</sub> Nanoparticles and the Coordination Compound of Sr(II) with Polydentat Ligand

  • Ciloci A.A.
  • Bulimaga V.P.
  • Clapco S.F.
  • Labliuc S.V.
  • Dvornina E.G.
Ciloci A.A.
Bulimaga V.P.
Clapco S.F.
Labliuc S.V.
Dvornina E.G.

Abstract

UDC 579.66

DOI  https://doi.org/10.52577/eom.2024.60.6.63

 

The stimulatory effect of TiO2 nanoparticles and of the coordination compound of Sr(II) with a polydentate ligand on the amylase activity was established under submerged cultivation of the micromycete strain Aspergillus niger CNMN FD 06. After purification by gel-filtration on the column of PAD-10 and the ion exchange chromatography on the Hi-TrapTMQ column, an increase in the specific activity of α-amylase in the case of the variants with the addition of TiO2 nanoparticles and coordination compound of Sr(II) up to 15523 and 14656 U/mg, respectively, compared to the control version 12037 U/mg. The yield of α-amylases after purification was also higher, with an increase of 31 and 20%, respectively, compared to that of the control variant. The SDS-PAGE analysis of the isolated proteins revealed the presence of two bands in the active fractions with an apparent molecular mass equal to 65 and 40 kDa, with a more abundant band with a molecular mass of 65 kDa in the case of the preparation obtained with the addition of the Sr(II) coordination compound.

 

Keywords: α-amylase activity, Aspergillus niger fungi, stimulants, purification, SDS-PAGE electrophoresis.

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