Rezumat
CZU 621.372:577.151
Micromycete Aspergillus niger 33-19 CNMN FD 02A standard cultivated and exposed to λ = 7.1 millimeter waves radiation has been used to obtain two enzyme preparations with the 10x degree of purity, with a 29.9% difference of the α-amylase activity in irradiated preparation but with a similar specific activity of protein of 138.3 U/mg and 141.0 U/mg. The α-amylase purification through gel filtration and ion exchange chromatography has resulted in the increase of 8.9 times of the specific activity for the control and of 8.2 times for the irradiated samples, with the yields of 25% and 17%, respectively. The SDS-PAGE of proteins isolated from initial preparations indicates the expression of new polypeptide of 45 kDa in the irradiated samples. Polypeptide spectrum of α-amylase from both preparations, purified through ion chromatography, shows two unchanged polypeptide bands of 66 and 40.5 kDa, identified as α-amylase isomorphs.
Keywords: millimeter-range electromagnetic radiation, fungi, Aspergillus niger, α-amylase purification.